Crystal structure of penicillin G acylase from the bro1 mutant strain ofprovidencia rettgeri
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منابع مشابه
Crystal structure of penicillin G acylase from the Bro1 mutant strain of Providencia rettgeri.
Penicillin G acylase is an important enzyme in the commercial production of semisynthetic penicillins used to combat bacterial infections. Mutant strains of Providencia rettgeri were generated from wild-type cultures subjected to nutritional selective pressure. One such mutant, Bro1, was able to use 6-bromohexanamide as its sole nitrogen source. Penicillin acylase from the Bro1 strain exhibited...
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The penicillin G acylase genes from the Proteus rettgeri wild type and from a hyperproducing mutant which is resistant to succinate repression were cloned in Escherichia coli K-12. Expression of both wild-type and mutant P. rettgeri acylase genes in E. coli K-12 was independent of orientation in the cloning vehicle and apparently resulted from recognition in E. coli of the P. rettgeri promoter ...
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Two isoforms of the heterodimeric enzyme penicillin G acylase (EC 3.5.1.1 I ) from Providencia rettgeri ATCC 31052 (strain Brol) were purified to near homogeneity. The isoforms exhibited comparable enzymatic a tivities but differed slightly in the molecular weight and PI of their respective a-subunit. The origin of this difference was traced to the partial conversion of the N-terminal Gln of th...
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A new crystal form of penicillin acylase (penicillin amidohydrolase, E.C. 3.5.1.11) from Escherichia coli W (ATCC 11105) is reported. The crystals were grown using a combination of hanging-drop and streak-seeding methods. The crystals are in the monoclinic space group P2(1) with cell dimensions a = 51.52, b = 131.95, c = 64.43 A, beta = 106.12 degrees. There is one heterodimer in the asymmetric...
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Penicillin G acylase (PGA; EC 3.5.1.11) is a hydrolytic enzyme that acts on the side chains of penicillin G, cephalosporin G and related ntibiotics to produce the -lactam antibiotic intermediates 6-amino penicillanic acid (6-APA) and 7-amino des-acetoxy cephalosporanic acid 7-ADCA), with phenyl acetic acid (PAA) as a common by-product. These antibiotic intermediates are among the potential buil...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1999
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.8.10.1971